Specifications

  • Transmission
    Excitation & Emission Filters: Tavg ≥ 96% over passband
  • Reflection
    Dichroic Beamsplitters: Ravg ≥ 98% over Excitation passband
  • Blocking
    ODavg ≥ 6 out of band, 300 – 900 nm ODavg ≥ 10 over corresponding EX/EM passband (verified to OD8 avg as a paired set) ODabs ≥ 4 EX/EM crossover ODavg ≥ 3 over 900 – 1100 nm
  • Angle of Incidence
    Excitation & Emission Filters: 0-5°
    Dichroic Beamsplitters: 45±1.5°
  • Parallelism
    Excitation & Emission Filters: ≤ 10 arcseconds
    Dichroic Beamsplitters: ≤ 5 arcsecond
  • Material
    Excitation & Emission Filters: BOROFLOAT® 33
    Dichroic Beamsplitters: Fused Silica
  • Dimensions
    Excitation & Emission Filters: 25 mm
    Dichroic Beamsplitters: 25.2 x 35.6 mm
  • Surface Quality
    60-40 over clear aperture

Features

ODiate Filter Manufacturing & Metrology

ODiate Fluorescence Filter Sets are manufactured using advanced thin-film deposition systems ensures optical filters achieve excellent transmission and reflection accuracy. ODiate filter specifications are verified utilizing commercial spectrophotometric tools in addition to Newport’s proprietary custom in-house tools to measure edge steepness resolution, and deep blocking to optical density 8 [-log10(T)].

Optimized Coatings and Engineering Expertise

Newport pushed the boundaries with the introduction of our ODiate Fluorescence Filter Sets, to allow customers to fully optimize their system performance.  Our expert engineering team has been involved from the first stage of design to ensure that we exceed your requirements and continues through the entire process of continual production deliveries. 

Environmental Stability and Physical Durability

The coating systems used to produce ODiate filters use a dense sputtering process to provide robust environmental reliability to meet the highest demands for thin-film coatings. The resulting highly durable coatings meet or exceed testing procedures including humidity, thermal shock, adhesion, chemical resistance, and moderate abrasion per U.S. Military and international standards.

Set Components

Each ODiate® Fluorescence Filter Set contains one each of the filters shown in the table below. Please see the individual filter pages for complete specifications and a list of other Fluorophores covered by the filter set.

Set Primary Fluorophore Excitation Filter Dichroic Beamsplitter Emission Filter
OD8-FFS2005 DAPI 379.5 nm Center 414 nm Cut-on 450 nm Center
OD8-FFS2010 GFP 474 nm Center 498 nm Cut-on 523 nm Center
OD8-FFS2015 FITC 482.5 nm Center 508.5 nm Cut-on 537 nm Center
OD8-FFS2020 TRITC 539 nm Center 560.5 nm Cut-on 593 nm Center
OD8-FFS2025 mCherry & TexasRed 564.5 nm Center 596 nm Cut-on 629 nm Center
OD8-FFS2030 Cy5™ 626 nm Center 658.5 nm Cut-on 692.5 nm Center

Resources

Literature

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